D-Aspartic Acid – does it really work?

Hard Rhino DAAThis article is about the science behind D-Aspartic Acid (DAA). To read more about DAA based stack click here.

D-Aspartic acid is an endogenous (meaning that it is produced by the body) amino acid which has been found in the nervous and endocrine (i.e. hormone producing) tissues of both animals and humans. It is one of 20 building blocks of proteins, called proteinogenic amino acids. D-Aspartic acid was discovered in 1827 by a French pharmacist Plisson, by boiling asparagus and treating it with hydrochloric acid – thus the name.

D-Aspartic acid is present in the brain tissue in the last stage of embryo development and shortly after birth of mammals, suggesting that it takes part in the nervous system development. Also a high concentration of the d-aspartic acid can be found in the testes where it plays a role in sperm production.

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D-Aspartic Acid ist the d-form of the amino acid aspartic acid. The l-form of all proteinogenic amino acids build tissues, the d-form is not a building block but a signaling molecule. In specific tissues of the body (testes, pituitary gland, and hypothalamus, all responsible for hormone production), the non-essential amino acid l-aspartic acid (l-form) is converted by a d-aspartate racemase and becomes the d-form, a racemate isomer of the l-form. The d-aspartic acid supplements contain thus the form of the aspartic acid responsible for increasing testosterone levels.

How does D-Aspartic Acid work?

D-Aspartic acid increases natural testosterone production and release, by increasing the release of gonadotrophin and luteinizing hormone. DAA triggers the release of the GnRH (gonadotropin-releasing hormone that informs the pituitary that testosterone concentration in the blood is falling), oxytocin and vasopressin (the “love hormones”), the luteinizing hormone (LH) and the growth hormone (GH) from the neuroendocrine axis (the hypothalamus and the pituitary gland), and testosterone from the testes. The pituary gland and the testes have the ability to trap circulating d-aspartic acid.

Natural increased release of GnRH and LH resulting in increased testosterone levels can be observed from puberty only to mid-twenties. Men after 30 experience lowered testosterone levels, which leads to a lowered libido, increased body fat levels, problems with sleep, decreased recovery ability, and decreased ability to build up muscle. In other words, DAA supplement causes you body to produce more testosterone as if you were younger and helps to get rid of the above mentioned symptoms. The deciding advantage of d-aspartic acid over direct testosterone supplementation (e.g. with steroids) is that the testosterone is produced by your body and not delivered from outside, which could interfere with body’s own testosterone production.

Increased testosterone levels triggered by d-aspartic acid benefit sexual performance and muscle growth. Being a male sex hormone testosterone enhances libido and erection quality, intensifies perceived orgasm and extends duration of intercourse. Testosterone makes your energy level rise, low T is responsible for depressions and lowered life satisfaction. By elevating testosterone level, d-aspartic acid makes your workouts more effective and helps you build muscle.

Stimulation with d-aspartic acid increases cyclic adenosine monophosphate (cAMP) in the receiving nerves. cAMP is a cell-regulating compound, probably the most important one. It plays an important role in the cellular reaction on hormones.
Which benefits does increased cAMP have for your body? Among others reactions it increases the force of contraction of the heart muscle, helps the arteries and other smooth muscles to relax, increases the secretion of insulin and increased lipolysis (fat destruction). In other words increased cAMP makes your workouts easier and helps you to burn fat.

D-Aspartic acid has been suggested to help promote normal GABA levels, and dopamine levels, acting anti-depressant. Another benefit of d-aspartic acid is its ability to increase nitric oxide (NO2, NO3) production and its blood level, promoting fast recovery after workouts.

Has D-Aspartic Acid been tested?

Unlike many other testosterone boosters, there is a scientific confirmation that DAA supplements increase testosterone levels by humans. A study has been conducted on human volunteers and on rats. 23 men between 27 and 37 have taken a DAA supplement for 12 days. The testosterone and LH levels have been significantly increased in comparison with a control group who took placebo (a solution of salt). The increase of luteinizing hormone was 33%, the increase of testosterone – a whooping 42% in only 12 days! Similar results have been observed with rats. An elevated level of testosterone in blood remained even three days after suspension of the treatment, probably due to the concentration of d-aspartic-acid trapped in tissues. It is noteworthy that this clinical research has been conducted on healthy men with normal testorone levels, not hypogonadal men.

A more recent research conducted 2014 on athletes have shown that d-aspartic acid can also let your testosterone levels drop. The subjects who had been given 6 gram a day experienced a decrease of the blood testosterone, while the subjects consuming only 3 gram a day didn’t suffer from such side effect. Lesson learned – never take more than the safe 3 gram DAA a day.

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A word of caution for the ladies – the research has been performed only on men. Another study gives clues how a supplementation of d-aspartic acid works on females – it increased the levels of estrogens. Probably DAA supplements should not be taken by women. However the study mentioned involved only animals (lizards), so the results should be taken with a pinch of salt.

DAA Supplements

D-Aspartic acid is one of the newest natural testosterone boosters available on the market. You can buy DAA supplement in form of pure DAA or stacked with other testosterone boosters, which is convenient but requires you to do a little research to make sure that you take only supplements you want to take. It is a good idea to stack DAA with other testosterone boosters that don’t already contain DAA.

Pure DAA is available in form of DAA powder, manufactured by e.g. AI Sports Nutrition, Allmax, Primaforce or DAA capsules, manufactured by e.g. AI Sports Nutrition, Performance Edge.
(Read more about DAA Supplement here.)

D-Aspartic Acid Dosage

The DAA dosage used in the above mentioned study was 3 gram taken in the morning. The manufacturer of the d-aspartic acid supplement Primaforce suggests a loading phase of five days, with 3 grams applied in the morning and another 3 grams in the evening. At the moment there are no side effects reported with the use of d-aspartic acid. However as with all testosterone boosters I would suggest you to cycle it to avoid building up a tolerance. Another manufacturer of the d-aspartic acid supplement, Allmax, suggests a cycle 2 weeks on and 1 week off the supplement.
(Read more about DAA Dosage here.)